Coding
Part:BBa_K2619004:Design
Designed by: shiyu liu Group: iGEM18_XJTLU-CHINA (2018-09-08)
19kDa nano luciferase/nanoLuc
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 230
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 46
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
no specific considerations
Source
INOUYE, S., SATO, J.-I., SAHARA-MIURA, Y., YOSHIDA, S., KURAKATA, H. & HOSOYA, T. 2013. C6-Deoxy coelenterazine analogues as an efficient substrate for glow luminescence reaction of nanoKAZ: The mutated catalytic 19kDa component of Oplophorus luciferase. Biochemical and Biophysical Research Communications, 437, 23-28.